Endo-β-1,4-glucanases impact plant cell wall development by influencing cellulose crystallization.
Identifieur interne : 001D94 ( Main/Exploration ); précédent : 001D93; suivant : 001D95Endo-β-1,4-glucanases impact plant cell wall development by influencing cellulose crystallization.
Auteurs : Magdalena Glass [Canada] ; Sarah Barkwill ; Faride Unda ; Shawn D. MansfieldSource :
- Journal of integrative plant biology [ 1744-7909 ] ; 2015.
Descripteurs français
- KwdFr :
- ADN bactérien (génétique), Arabidopsis (anatomie et histologie), Arabidopsis (métabolisme), Cellulase (métabolisme), Cellules végétales (métabolisme), Cellulose (métabolisme), Coloration et marquage (MeSH), Cristallisation (MeSH), Gènes de plante (MeSH), Interférence par ARN (MeSH), Mutagenèse par insertion (génétique), Métabolisme glucidique (MeSH), Paroi cellulaire (métabolisme), Phylogenèse (MeSH), Populus (croissance et développement), Populus (enzymologie), Populus (génétique), Régulation négative (MeSH).
- MESH :
- anatomie et histologie : Arabidopsis.
- croissance et développement : Populus.
- enzymologie : Populus.
- génétique : ADN bactérien, Mutagenèse par insertion, Populus.
- métabolisme : Arabidopsis, Cellulase, Cellules végétales, Cellulose, Paroi cellulaire.
- Coloration et marquage, Cristallisation, Gènes de plante, Interférence par ARN, Métabolisme glucidique, Phylogenèse, Régulation négative.
English descriptors
- KwdEn :
- Arabidopsis (anatomy & histology), Arabidopsis (metabolism), Carbohydrate Metabolism (MeSH), Cell Wall (metabolism), Cellulase (metabolism), Cellulose (metabolism), Crystallization (MeSH), DNA, Bacterial (genetics), Down-Regulation (MeSH), Genes, Plant (MeSH), Mutagenesis, Insertional (genetics), Phylogeny (MeSH), Plant Cells (metabolism), Populus (enzymology), Populus (genetics), Populus (growth & development), RNA Interference (MeSH), Staining and Labeling (MeSH).
- MESH :
- chemical , genetics : DNA, Bacterial.
- chemical , metabolism : Cellulase, Cellulose.
- anatomy & histology : Arabidopsis.
- enzymology : Populus.
- genetics : Mutagenesis, Insertional, Populus.
- growth & development : Populus.
- metabolism : Arabidopsis, Cell Wall, Plant Cells.
- Carbohydrate Metabolism, Crystallization, Down-Regulation, Genes, Plant, Phylogeny, RNA Interference, Staining and Labeling.
Abstract
Cell walls are vital to the normal growth and development of plants as they protect the protoplast and provide rigidity to the stem. Here, two poplar and Arabidopsis orthologous endoglucanases, which have been proposed to play a role in secondary cell wall development, were examined. The class B endoglucanases, PtGH9B5 and AtGH9B5, are secreted enzymes that have a predicted glycosylphosphatidylinositol anchor, while the class C endoglucanases, PtGH9C2 and AtGH9C2, are also predicted to be secreted but instead contain a carbohydrate-binding module. The poplar endoglucanases were expressed in Arabidopsis using both a 35S promoter and the Arabidopsis secondary cell wall-specific CesA8 promoter. Additionally, Arabidopsis t-DNA insertion lines and an RNAi construct was created to downregulate AtGH9C2 in Arabidopsis. All of the plant lines were examined for changes in cell morphology and patterning, growth and development, cell wall crystallinity, microfibril angle, and proportion of cell wall carbohydrates. Misregulation of PtGH9B5/AtGH9B5 resulted in changes in xylose content, while misregulation of PtGH9C2/AtGH9C2 resulted in changes in crystallinity, which was inversely correlated with changes in plant height and rosette diameter. Together, these results suggest that these endoglucanases affect secondary cell wall development by contributing to the cell wall crystallization process.
DOI: 10.1111/jipb.12353
PubMed: 25756224
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Mansfield</name></author></analytic><series><title level="j">Journal of integrative plant biology</title><idno type="eISSN">1744-7909</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (anatomy & histology)</term><term>Arabidopsis (metabolism)</term><term>Carbohydrate Metabolism (MeSH)</term><term>Cell Wall (metabolism)</term><term>Cellulase (metabolism)</term><term>Cellulose (metabolism)</term><term>Crystallization (MeSH)</term><term>DNA, Bacterial (genetics)</term><term>Down-Regulation (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Mutagenesis, Insertional (genetics)</term><term>Phylogeny (MeSH)</term><term>Plant Cells (metabolism)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>RNA Interference (MeSH)</term><term>Staining and Labeling (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (génétique)</term><term>Arabidopsis (anatomie et histologie)</term><term>Arabidopsis (métabolisme)</term><term>Cellulase (métabolisme)</term><term>Cellules végétales (métabolisme)</term><term>Cellulose (métabolisme)</term><term>Coloration et marquage (MeSH)</term><term>Cristallisation (MeSH)</term><term>Gènes de plante (MeSH)</term><term>Interférence par ARN (MeSH)</term><term>Mutagenèse par insertion (génétique)</term><term>Métabolisme glucidique (MeSH)</term><term>Paroi cellulaire (métabolisme)</term><term>Phylogenèse (MeSH)</term><term>Populus (croissance et développement)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Régulation négative (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulase</term><term>Cellulose</term></keywords><keywords scheme="MESH" qualifier="anatomie et histologie" xml:lang="fr"><term>Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Mutagenesis, Insertional</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>Mutagenèse par insertion</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arabidopsis</term><term>Cell Wall</term><term>Plant Cells</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arabidopsis</term><term>Cellulase</term><term>Cellules végétales</term><term>Cellulose</term><term>Paroi cellulaire</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Carbohydrate Metabolism</term><term>Crystallization</term><term>Down-Regulation</term><term>Genes, Plant</term><term>Phylogeny</term><term>RNA Interference</term><term>Staining and Labeling</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Coloration et marquage</term><term>Cristallisation</term><term>Gènes de plante</term><term>Interférence par ARN</term><term>Métabolisme glucidique</term><term>Phylogenèse</term><term>Régulation négative</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cell walls are vital to the normal growth and development of plants as they protect the protoplast and provide rigidity to the stem. Here, two poplar and Arabidopsis orthologous endoglucanases, which have been proposed to play a role in secondary cell wall development, were examined. The class B endoglucanases, PtGH9B5 and AtGH9B5, are secreted enzymes that have a predicted glycosylphosphatidylinositol anchor, while the class C endoglucanases, PtGH9C2 and AtGH9C2, are also predicted to be secreted but instead contain a carbohydrate-binding module. The poplar endoglucanases were expressed in Arabidopsis using both a 35S promoter and the Arabidopsis secondary cell wall-specific CesA8 promoter. Additionally, Arabidopsis t-DNA insertion lines and an RNAi construct was created to downregulate AtGH9C2 in Arabidopsis. All of the plant lines were examined for changes in cell morphology and patterning, growth and development, cell wall crystallinity, microfibril angle, and proportion of cell wall carbohydrates. Misregulation of PtGH9B5/AtGH9B5 resulted in changes in xylose content, while misregulation of PtGH9C2/AtGH9C2 resulted in changes in crystallinity, which was inversely correlated with changes in plant height and rosette diameter. Together, these results suggest that these endoglucanases affect secondary cell wall development by contributing to the cell wall crystallization process. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25756224</PMID><DateCompleted><Year>2016</Year><Month>01</Month><Day>05</Day></DateCompleted><DateRevised><Year>2015</Year><Month>04</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1744-7909</ISSN><JournalIssue CitedMedium="Internet"><Volume>57</Volume><Issue>4</Issue><PubDate><Year>2015</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Journal of integrative plant biology</Title><ISOAbbreviation>J Integr Plant Biol</ISOAbbreviation></Journal><ArticleTitle>Endo-β-1,4-glucanases impact plant cell wall development by influencing cellulose crystallization.</ArticleTitle><Pagination><MedlinePgn>396-410</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/jipb.12353</ELocationID><Abstract><AbstractText>Cell walls are vital to the normal growth and development of plants as they protect the protoplast and provide rigidity to the stem. Here, two poplar and Arabidopsis orthologous endoglucanases, which have been proposed to play a role in secondary cell wall development, were examined. The class B endoglucanases, PtGH9B5 and AtGH9B5, are secreted enzymes that have a predicted glycosylphosphatidylinositol anchor, while the class C endoglucanases, PtGH9C2 and AtGH9C2, are also predicted to be secreted but instead contain a carbohydrate-binding module. The poplar endoglucanases were expressed in Arabidopsis using both a 35S promoter and the Arabidopsis secondary cell wall-specific CesA8 promoter. Additionally, Arabidopsis t-DNA insertion lines and an RNAi construct was created to downregulate AtGH9C2 in Arabidopsis. All of the plant lines were examined for changes in cell morphology and patterning, growth and development, cell wall crystallinity, microfibril angle, and proportion of cell wall carbohydrates. Misregulation of PtGH9B5/AtGH9B5 resulted in changes in xylose content, while misregulation of PtGH9C2/AtGH9C2 resulted in changes in crystallinity, which was inversely correlated with changes in plant height and rosette diameter. Together, these results suggest that these endoglucanases affect secondary cell wall development by contributing to the cell wall crystallization process. </AbstractText><CopyrightInformation>© 2015 Institute of Botany, Chinese Academy of Sciences.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Glass</LastName><ForeName>Magdalena</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Wood Science, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Barkwill</LastName><ForeName>Sarah</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Unda</LastName><ForeName>Faride</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Mansfield</LastName><ForeName>Shawn D</ForeName><Initials>SD</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China (Republic : 1949- )</Country><MedlineTA>J Integr Plant Biol</MedlineTA><NlmUniqueID>101250502</NlmUniqueID><ISSNLinking>1672-9072</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C036483">T-DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber><NameOfSubstance UI="D002480">Cellulase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D050260" MajorTopicYN="N">Carbohydrate Metabolism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002480" MajorTopicYN="N">Cellulase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003460" MajorTopicYN="N">Crystallization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015536" MajorTopicYN="N">Down-Regulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016254" MajorTopicYN="N">Mutagenesis, Insertional</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D059828" MajorTopicYN="N">Plant Cells</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D034622" MajorTopicYN="N">RNA Interference</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013194" MajorTopicYN="N">Staining and Labeling</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cellulose</Keyword><Keyword MajorTopicYN="N">cell walls</Keyword><Keyword MajorTopicYN="N">crystallinity</Keyword><Keyword MajorTopicYN="N">endoglucanase</Keyword><Keyword MajorTopicYN="N">expansion</Keyword><Keyword MajorTopicYN="N">glucose</Keyword><Keyword MajorTopicYN="N">xylose</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>11</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>03</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>3</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>3</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>1</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25756224</ArticleId><ArticleId IdType="doi">10.1111/jipb.12353</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Colombie-Britannique </li></region><settlement><li>Vancouver</li></settlement><orgName><li>Université de la Colombie-Britannique</li></orgName></list><tree><noCountry><name sortKey="Barkwill, Sarah" sort="Barkwill, Sarah" uniqKey="Barkwill S" first="Sarah" last="Barkwill">Sarah Barkwill</name><name sortKey="Mansfield, Shawn D" sort="Mansfield, Shawn D" uniqKey="Mansfield S" first="Shawn D" last="Mansfield">Shawn D. Mansfield</name><name sortKey="Unda, Faride" sort="Unda, Faride" uniqKey="Unda F" first="Faride" last="Unda">Faride Unda</name></noCountry><country name="Canada"><region name="Colombie-Britannique "><name sortKey="Glass, Magdalena" sort="Glass, Magdalena" uniqKey="Glass M" first="Magdalena" last="Glass">Magdalena Glass</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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